PURPOSE: To produce a pepsinogen in high efficiency, by linking a structure gene originated from other animal cell to the down-stream side of a plasmid containing a specific base sequence and introducing the resultant plasmid vector into Bacillus brevis.
CONSTITUTION: The 5'-region or a 150,000 protein gene of a plasmid pCWPI is cut with AluI-AluI and BamHI linker is linked with T4 ligase. Said fragment is linked with T4 ligase to a fragment produced by removing 5'-terminal phosphoric acid group from BamHI-BglII fragment of plasmide pHTI and the linked product is introduced into Bacillus brevis 47-5 to obtain plasmid pNU100. A HindIII-EcoRI fragment of plasmid pASI containing pepsinnnogen gene originated preferably from swine is inserted into the BamHI cut site of said plasmid to obtain a plasmid vector. A Bacillus brevis is transformed with said vector to produce pepsinogen.
UDAKA JUZO
TSUKAGOSHI NORIHIRO
YAMAGATA HIDEO
TAKAHASHI KENJI
