Title:
ウイルス不活化成長因子を含有する且つPDGF及びVEGF枯渇の血小板ライセ-トとその製造方法
Document Type and Number:
Japanese Patent JP2013528173
Kind Code:
A
Abstract:
There is an increasing interest in human platelet extracts rich in growth factors (PGF) both for wound healing and for stem cell expansion. The present invention concerns a virally-inactivated growth factors-containing platelet lysate depleted of PDGF and VEGF, which is preferably enriched in TGF, IGF and EGF-rich. The present invention further concerns a method for obtaining such a platelet lysate, comprising the steps of contacting a starting platelet concentrate with a solvent and/or a detergent, incubating said starting platelet concentrate with the solvent and/or detergent for a period of at least 5 minutes to 6 hours, at a pH maintained in a range from about 6.0 to about 9.0, and at a temperature within the range of from 2°C to 50°C, optionally removing the solvent and/or the detergent by oil extraction and obtaining an aqueous protein phase; and incubating the solvent and/or detergent-tretaed platelet concentrate or the aqueous protein phase with charcoal.
Inventors:
Thierry Burneuf
Sue Wen Yao
Sue Wen Yao
Application Number:
JP2013511778A
Publication Date:
July 08, 2013
Filing Date:
May 25, 2011
Export Citation:
Assignee:
GWOWEI TECHNOLOGY CO., LTD
International Classes:
C07K14/475; A61K8/98; A61K35/14; A61K35/19; A61L27/00; A61P19/00; C07K1/14; C07K1/16; C07K1/34; C07K14/485; C07K14/495; C12N5/077
Attorney, Agent or Firm:
Kosuke Suzuki