Title:
POLYPEPTIDE CONTAINING DNA-BINDING DOMAIN
Document Type and Number:
Japanese Patent JP2015033365
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a polypeptide that satisfies both of high function of a functional domain and high recognition specificity to DNA sequences, can maximize a desired function safely at a high rate, and furthermore can be produced with simple operation.SOLUTION: There are provided: an artificial nuclease that contains a DNA-binding domain and a functional domain, the domains being bonded together via a polypeptide consisting of 35-55 amino acids, wherein amino acids at two positions in DNA-binding modules contained in the DNA-binding domain show a repetition pattern that is different in each four DNA-binding modules; a vector for expressing the artificial nuclease; a vector library for constructing the vector; and a vector set for constructing the vector library.
Inventors:
YAMAMOTO TAKU
SAKUMA TETSUSHI
OCHIAI HIROSHI
MATSUURA SHINYA
MIYAMOTO TATSUO
SAKUMA TETSUSHI
OCHIAI HIROSHI
MATSUURA SHINYA
MIYAMOTO TATSUO
Application Number:
JP2013166768A
Publication Date:
February 19, 2015
Filing Date:
August 09, 2013
Export Citation:
Assignee:
UNIV HIROSHIMA
International Classes:
C12N15/09; C07K14/195; C07K19/00; C12N9/22; C40B40/08
Domestic Patent References:
| JP2013529083A | 2011-09-20 | |||
| JP2013094148A | 2013-05-20 |
Other References:
JPN6014033141; Ｔｅｔｓｕｓｈｉ Ｓａｋｕｍａ ｅｔ ａｌ．: 'Ａｄｖａｎｃｅｄ ｍｅｔｈｏｄｓ ｆｏｒ ｔｈｅ ｃｏｎｓｔｒｕｃｔｉｏｎ， ｅｖａｌｕａｔｉｏｎ ａｎｄ ａｐｐｌｉｃａｔｉｏｎ ｏｆ ＴＡＬＥＮｓ' Ｊｏｉｎｔ Ｍｅｅｔｉｎｇ ｏｆ Ｔｈｅ ４５ｔｈ Ａｎｎｕａｌ Ｍｅｅｔｉｎｇ ｏｆ ｔｈｅ Ｊａｐａｎｅｓｅ Ｓｏｃｉｅｔｙ ｏｆ Ｄｅｖｅｌｏｐｍｅｎｔａｌ Ｂｉｏｌｏｇｉｓｔｓ ＆ Ｔｈｅ ６ , 20120427, ｐ． ３０３， ＪＹＳＳ２-２２（Ｐ１-１２８）
JPN7014002386; Ｔｅｔｓｕｓｈｉ Ｓａｋｕｍａ ａｎｄ Ｔａｋａｓｈｉ Ｙａｍａｍｏｔｏ: 'Ｐｌａｔｉｎｕｍ Ｇａｔｅ ＴＡＬＥＮ： Ｅｓｔａｂｌｉｓｈｍｅｎｔ ｏｆ ｈｉｇｈｌｙ-ａｃｔｉｖｅ ＴＡＬＥＮ ｃｏｎｓｔｒｕｃｔｉｏｎ ｓｙｓｔｅｍ' 第４６回日本発生生物学会予稿集 , 20130515, ＴＷ０１-４
JPN6014033146; Ｓａｔｏｓｈｉ Ｏｔａ ｅｔ ａｌ．: 'Ｅｆｆｉｃｉｅｎｔ ｉｄｅｎｔｉｆｉｃａｔｉｏｎ ｏｆ ＴＡＬＥＮ-ｍｅｄｉａｔｅｄ ｇｅｎｏｍｅ ｍｏｄｉｆｉｃａｔｉｏｎｓ ｕｓｉｎｇ ｈｅｔｅｒｏｄｕｐｌｅｘ ｍｏｂｉｌｉｔｙ ａｓｓａｙｓ' Ｇｅｎｅｓ ｔｏ Ｃｅｌｌｓ Ｖｏｌ． １８， Ｎｏ． ６, 201306, ｐ． ４５０-４５８
JPN6014033148; Ｊｅｆｆｒｅｙ Ｃ Ｍｉｌｌｅｒ ｅｔ ａｌ．: 'Ａ ＴＡＬＥ ｎｕｃｌｅａｓｅ ａｒｃｈｉｔｅｃｔｕｒｅ ｆｏｒ ｅｆｆｉｃｉｅｎｔ ｇｅｎｏｍｅ ｅｄｉｔｉｎｇ' Ｎａｔｕｒｅ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ Ｖｏｌ． ２９, 2011, ｐ． １４３-１４８
JPN6014033150; Ｔｈｏｍａｓ Ｇａｊ ｅｔ ａｌ．: 'ＺＦＮ， ＴＡＬＥＮ， ａｎｄ ＣＲＩＳＰＲ／Ｃａｓ-ｂａｓｅｄ ｍｅｔｈｏｄｓ ｆｏｒ ｇｅｎｏｍｅ ｅｎｇｉｎｅｅｒｉｎｇ' Ｔｒｅｎｄｓ ｉｎ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ Ｖｏｌ． ３１， Ｎｏ． ７, 201307, ｐ． ３９７-４０５
JPN7014002387; Ｔｏｍａｓ Ｃｅｒｍａｋ ｅｔ ａｌ．: 'Ｅｆｆｉｃｉｅｎｔ ｄｅｓｉｇｎ ａｎｄ ａｓｓｅｍｂｌｙ ｏｆ ｃｕｓｔｏｍ ＴＡＬＥＮ ａｎｄ ｏｔｈｅｒ ＴＡＬ ｅｆｆｅｃｔｏｒ-ｂａｓｅｄ ｃｏｎｓｔｒｕｃｔｓ ｆｏｒ ＤＮＡ ｔａｒｇｅｔ' Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓｅａｒｃｈ Ｖｏｌ． ３９， Ｎｏ． １２, 2011, ｅ８２
JPN6014033155; Ｔｅｔｓｕｓｈｉ Ｓａｋｕｍａ ｅｔ ａｌ．: 'Ｒｅｐｅａｔｉｎｇ ｐａｔｔｅｒｎ ｏｆ ｎｏｎ-ＲＶＤ ｖａｒｉａｔｉｏｎｓ ｉｎ ＤＮＡ-ｂｉｎｄｉｎｇ ｍｏｄｕｌｅｓ ｅｎｈａｎｃｅｓ ＴＡＬＥＮ ａｃｔｉｖｉｔｙ' Ｓｃｉｅｎｔｉｆｉｃ Ｒｅｐｏｒｔｓ Ｖｏｌ． ３, 20131129, Ａｒｔｉｃｌｅ ｎｕｍｂｅｒ： ３３７９
JPN6014033153; Ｊｅｎｓ Ｂｏｃｈ ａｎｄ Ｕｌｌａ Ｂｏｎａｓ: 'Ｘａｎｔｈｏｍｏｎａｓ ＡｖｒＢｓ３ Ｆａｍｉｌｙ-Ｔｙｐｅ ＩＩＩ Ｅｆｆｅｃｔｏｒｓ： Ｄｉｓｃｏｖｅｒｙ ａｎｄ Ｆｕｎｃｔｉｏｎ' Ａｎｎｕａｌ Ｒｅｖｉｅｗ ｏｆ Ｐｈｙｔｏｐａｔｈｏｌｏｇｙ Ｖｏｌ． ４８, 2010, ｐ． ４１９-４３６
JPN7014002386; Ｔｅｔｓｕｓｈｉ Ｓａｋｕｍａ ａｎｄ Ｔａｋａｓｈｉ Ｙａｍａｍｏｔｏ: 'Ｐｌａｔｉｎｕｍ Ｇａｔｅ ＴＡＬＥＮ： Ｅｓｔａｂｌｉｓｈｍｅｎｔ ｏｆ ｈｉｇｈｌｙ-ａｃｔｉｖｅ ＴＡＬＥＮ ｃｏｎｓｔｒｕｃｔｉｏｎ ｓｙｓｔｅｍ' 第４６回日本発生生物学会予稿集 , 20130515, ＴＷ０１-４
JPN6014033146; Ｓａｔｏｓｈｉ Ｏｔａ ｅｔ ａｌ．: 'Ｅｆｆｉｃｉｅｎｔ ｉｄｅｎｔｉｆｉｃａｔｉｏｎ ｏｆ ＴＡＬＥＮ-ｍｅｄｉａｔｅｄ ｇｅｎｏｍｅ ｍｏｄｉｆｉｃａｔｉｏｎｓ ｕｓｉｎｇ ｈｅｔｅｒｏｄｕｐｌｅｘ ｍｏｂｉｌｉｔｙ ａｓｓａｙｓ' Ｇｅｎｅｓ ｔｏ Ｃｅｌｌｓ Ｖｏｌ． １８， Ｎｏ． ６, 201306, ｐ． ４５０-４５８
JPN6014033148; Ｊｅｆｆｒｅｙ Ｃ Ｍｉｌｌｅｒ ｅｔ ａｌ．: 'Ａ ＴＡＬＥ ｎｕｃｌｅａｓｅ ａｒｃｈｉｔｅｃｔｕｒｅ ｆｏｒ ｅｆｆｉｃｉｅｎｔ ｇｅｎｏｍｅ ｅｄｉｔｉｎｇ' Ｎａｔｕｒｅ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ Ｖｏｌ． ２９, 2011, ｐ． １４３-１４８
JPN6014033150; Ｔｈｏｍａｓ Ｇａｊ ｅｔ ａｌ．: 'ＺＦＮ， ＴＡＬＥＮ， ａｎｄ ＣＲＩＳＰＲ／Ｃａｓ-ｂａｓｅｄ ｍｅｔｈｏｄｓ ｆｏｒ ｇｅｎｏｍｅ ｅｎｇｉｎｅｅｒｉｎｇ' Ｔｒｅｎｄｓ ｉｎ Ｂｉｏｔｅｃｈｎｏｌｏｇｙ Ｖｏｌ． ３１， Ｎｏ． ７, 201307, ｐ． ３９７-４０５
JPN7014002387; Ｔｏｍａｓ Ｃｅｒｍａｋ ｅｔ ａｌ．: 'Ｅｆｆｉｃｉｅｎｔ ｄｅｓｉｇｎ ａｎｄ ａｓｓｅｍｂｌｙ ｏｆ ｃｕｓｔｏｍ ＴＡＬＥＮ ａｎｄ ｏｔｈｅｒ ＴＡＬ ｅｆｆｅｃｔｏｒ-ｂａｓｅｄ ｃｏｎｓｔｒｕｃｔｓ ｆｏｒ ＤＮＡ ｔａｒｇｅｔ' Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓｅａｒｃｈ Ｖｏｌ． ３９， Ｎｏ． １２, 2011, ｅ８２
JPN6014033155; Ｔｅｔｓｕｓｈｉ Ｓａｋｕｍａ ｅｔ ａｌ．: 'Ｒｅｐｅａｔｉｎｇ ｐａｔｔｅｒｎ ｏｆ ｎｏｎ-ＲＶＤ ｖａｒｉａｔｉｏｎｓ ｉｎ ＤＮＡ-ｂｉｎｄｉｎｇ ｍｏｄｕｌｅｓ ｅｎｈａｎｃｅｓ ＴＡＬＥＮ ａｃｔｉｖｉｔｙ' Ｓｃｉｅｎｔｉｆｉｃ Ｒｅｐｏｒｔｓ Ｖｏｌ． ３, 20131129, Ａｒｔｉｃｌｅ ｎｕｍｂｅｒ： ３３７９
JPN6014033153; Ｊｅｎｓ Ｂｏｃｈ ａｎｄ Ｕｌｌａ Ｂｏｎａｓ: 'Ｘａｎｔｈｏｍｏｎａｓ ＡｖｒＢｓ３ Ｆａｍｉｌｙ-Ｔｙｐｅ ＩＩＩ Ｅｆｆｅｃｔｏｒｓ： Ｄｉｓｃｏｖｅｒｙ ａｎｄ Ｆｕｎｃｔｉｏｎ' Ａｎｎｕａｌ Ｒｅｖｉｅｗ ｏｆ Ｐｈｙｔｏｐａｔｈｏｌｏｇｙ Ｖｏｌ． ４８, 2010, ｐ． ４１９-４３６
Attorney, Agent or Firm:
Mitsuo Tanaka
Yamasaki 宏
Norifumi Tomita
Nobuhiro Nonaka
Yamasaki 宏
Norifumi Tomita
Nobuhiro Nonaka
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