Title:
POLYPEPTIDE OF HEPARAN SULFATE 6-O-SULFOTRANSFERASE AND DMA CAPABLE OF ENCODING THE SAME
Document Type and Number:
Japanese Patent JP3964982
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain the subject new DNA, comprising a base sequence capable of encoding a polypeptide of a glycosaminoglycan sulfotransferase and capable of mass-producing the enzyme useful for related research or the like of the structure and function of the glycosaminoglycan.
SOLUTION: This new DNA has a base sequence, coding a polypeptide of a glycosaminoglycan sulfotansferase, having an amino acid sequence represented by the formula and actions of selectively transferring a sulfate group from a sulfate group donor to the hydroxyl group at the 6-position in an N-sulfated glucosamine residue contained in a glycosaminoglycan which is a sulfate group acceptor, acting on a heparan sulfate or a completely desulfated, N-sulfated (CDSNS)-heparin without acting on chondroitin and chondroitin 4-sulfate, having 6-7 optimal reactional pH and 0.1-0.3 M optimal ionic strength when using NaCl, inhibited with dithiothreitol and adenosine-3',5'-diphosphate and activated with a protamine and is obtained by screening a cDNA library derived from a human fetal brain.
Inventors:
Hiroko Habuchi
Kobayashi
Kimata Koji
Hiromasa Suzuki
Masayuki Tanaka
Kobayashi
Kimata Koji
Hiromasa Suzuki
Masayuki Tanaka
Application Number:
JP11757098A
Publication Date:
August 22, 2007
Filing Date:
April 27, 1998
Export Citation:
Assignee:
Seikagaku Corporation
International Classes:
C12N15/09; C12N5/00; C12N5/10; C12N9/10; C12N15/00; C12R1/91; (IPC1-7): C12N15/09; C12N9/10; //C12N5/10; (C12N15/09; C12R1:91); (C12N9/10; C12R1:91); (C12N5/10; C12R1:91)
Other References:
J. Biol. Chem. (1995) vol.270, no.8, p.4172-4179
J. Biol. Chem. (1998 Apr 10) vol.273, no.15, p.9208-9213
J. Biol. Chem. (1998 Apr 10) vol.273, no.15, p.9208-9213
Attorney, Agent or Firm:
Yoshiyuki Kawaguchi
Hidemi Matsukura
Tsutomu Toyama
Hidemi Matsukura
Tsutomu Toyama