Title:
ミトコンドリアをターゲティングするポリペプチド
Document Type and Number:
Japanese Patent JP2009520488
Kind Code:
A
Abstract:
Methods for delivering non-mitochondrial proteins to mitochondria are provided. Also provided are nucleic acid constructs comprising a coding sequence encoding a DNA-binding polypeptide, fused to a mitochondrial targeting sequence (MTS) and a nuclear export signal (NES), and the encoded proteins. The construct successfully delivers DNA binding proteins to the mitochondrion. A chimeric methylase based on the above construct is successfully delivered to mitochondria, resulting in modification of mtDNA.
Inventors:
Mink zu, michael
Papworth, Monica A.
Crag, Aaron
Papworth, Monica A.
Crag, Aaron
Application Number:
JP2008546588A
Publication Date:
May 28, 2009
Filing Date:
December 19, 2006
Export Citation:
Assignee:
Medical research counsel
International Classes:
C12N15/09; A61K38/00; A61K48/00; A61P1/04; A61P3/10; A61P9/00; A61P9/10; A61P21/04; A61P25/16; A61P25/28; A61P27/00; A61P27/16; A61P43/00; C07K14/47; C07K19/00; C12N5/10
Domestic Patent References:
| JP2005229946A | 2005-09-02 |
Other References:
JPN6012011599; Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｓｙｍｐ． Ｓｅｒ． Ｖｏｌ． ４８, 2004, ｐ． ９９-１００
JPN6012011601; Ｇｅｎｅ Ｖｏｌ． ３６６, 20051117, ｐ． ２７-３８
JPN6012011603; Ｊ． Ｃｏｎｔｒｏｌ． Ｒｅｌｅａｓｅ Ｖｏｌ． ９８, 2004, ｐ． ３７９-３９３
JPN6012011597; Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓ． Ｖｏｌ． ３１， Ｎｏ． ２２, 2003, ｐ． ６４９３-６５０１
JPN6012011601; Ｇｅｎｅ Ｖｏｌ． ３６６, 20051117, ｐ． ２７-３８
JPN6012011603; Ｊ． Ｃｏｎｔｒｏｌ． Ｒｅｌｅａｓｅ Ｖｏｌ． ９８, 2004, ｐ． ３７９-３９３
JPN6012011597; Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓ． Ｖｏｌ． ３１， Ｎｏ． ２２, 2003, ｐ． ６４９３-６５０１
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita