Title:
POLYSACCHARIDE MODIFIED IMMUNOGLOBULIN HAVING REDUCED IMMUNOGENETIC CAPACITY OR IMPROVED DRUG KINETICS
Document Type and Number:
Japanese Patent JPH01163133
Kind Code:
A
Abstract:
PURPOSE: To obtain an immunoreactive composition comprising an immunoglobulin covalently bonded to a low-molecular weight polysaccharide or its antigen recognizing fragment and having reduced immunogen performance or improved medicine action. CONSTITUTION: A hydroxyl group of a polysaccharide having low-molecular weight, preferably a reactive hydroxyl group of dextran having 3,000-16,000 molecular weight, especially 6,000-16,000 molecular weight is oxidized into an active aldehyde group in the presence of an oxidizing agent, preferably sodium periodate to activate the polysaccharide and mixed with desired immunoglobulin in a proper solution (e.g. buffer solution having pH6.5-7.5) and aldehyde group of oxidized dextran is reacted with amino group of the immunoglobulin to form a Schiff base adduct. The Schiff base adduct is reduced with a proper reducing agent (cyanoboron hydride sodium) to provide the objective dextran- immunoglobulin adduct of substantially monomer.
Inventors:
ROBAATO SHISEEA FUANANI
Application Number:
JP28115888A
Publication Date:
June 27, 1989
Filing Date:
November 05, 1988
Export Citation:
Assignee:
HYBRITECH INC
International Classes:
A61K39/44; A61K45/00; A61P35/00; C07K14/00; C07K16/00; C07K19/00; C12N15/02; C12P21/08; G01N33/531; A61K39/395; G01N33/563; (IPC1-7): A61K39/395; A61K45/00; C07K3/08; C07K15/14; C07K15/22; C12N15/00; C12P21/00; G01N33/531; G01N33/563
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)