Title:
分娩後の哺乳動物の胎盤、その使用およびそれに由来する胎盤幹細胞
Document Type and Number:
Japanese Patent JP2004528021
Kind Code:
A
Abstract:
The present invention provides a method of extracting and recovering embryonic like stem cells, including, but not limited to pluripotent or multipotent stem cells, from an exsanguinated human placenta. A placenta is treated to move residual umbilical cord blood by perfusing an exsanguinated placenta, preferably with an anticoagulant solution, to flush out residual cells. The residual cells and perfusion liquid from the exsanguinated placenta are collected, and the embryonic-like stem cells are separated from the residual cells and perfusion liquid. The invention also provides a method of utilizing the isolated and perfused placenta as a bioreactor in which to propagate endogenous cells, including, but not limited to, embryonic-like stem cells. The invention also provides methods for propagation of exogenous cells in a placental bioreactor and collecting the propagated exogenous cells and bioactive molecules therefrom.
Inventors:
Hariri, Robert, Jay.
Application Number:
JP2002565070A
Publication Date:
September 16, 2004
Filing Date:
February 13, 2002
Export Citation:
Assignee:
Anthropogenesis Corporation
International Classes:
A61K35/14; A61K35/44; A61K48/00; A61M1/02; A61P3/00; A61P5/14; A61P7/06; A61P9/00; A61P9/10; A61P13/12; A61P17/02; A61P21/00; A61P25/00; A61P25/16; A61P25/28; A61P27/02; A61P27/06; A61P29/00; A61P43/00; C12M1/00; C12M1/34; C12M1/36; C12M3/00; C12N5/00; C12N5/073; C12N15/00; A61K35/12; (IPC1-7): C12N5/06; A61K35/14; A61K35/28; A61K35/44; A61K35/50; A61K48/00; A61P3/00; A61P5/14; A61P7/06; A61P9/00; A61P9/10; A61P13/12; A61P17/02; A61P21/00; A61P25/00; A61P25/16; A61P25/28; A61P27/02; A61P27/06; A61P29/00; A61P43/00; C12M1/00; C12M1/34; C12M1/36; C12M3/00
Other References:
JPN4007015122, Ｊ． Ｉｍｍｕｎｏｌ． Ｍｅｔｈｏｄｓ．， （１９９７）， ２０９， ［１］， ｐ．９３−１０４
JPN6009007243, Ｊ． Ｉｍｍｕｎｏｌ． Ｍｅｔｈｏｄｓ．， （１９９７）， ２０９， ［１］， ｐ．９３−１０４
JPN5003012766, Ｂｌｏｏｄ， （２００１．１１．１６）， ９８， ［１１］， ｐ．１４７Ｂ（Ａｂｓｔｒａｃｔ＃ ４２６０）
JPN6009007243, Ｊ． Ｉｍｍｕｎｏｌ． Ｍｅｔｈｏｄｓ．， （１９９７）， ２０９， ［１］， ｐ．９３−１０４
JPN5003012766, Ｂｌｏｏｄ， （２００１．１１．１６）， ９８， ［１１］， ｐ．１４７Ｂ（Ａｂｓｔｒａｃｔ＃ ４２６０）
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita
Ishii Teiji
Satoshi Fujita
Ishii Teiji