Title:
デング熱ウイルスのプレM/Mエピトープ、合成ペプチド、キメラタンパク質およびその用途
Document Type and Number:
Japanese Patent JP3647048
Kind Code:
B2
Abstract:
The present invention relates to five synthetic peptides of pre-M/M protein of Dengue-2 virus, corresponding to amino acid sequences 3-31, 45-67, 57-92, 69-93 and 103-124. The anti-peptide immune response was evaluated in mice. Recombinant fusion proteins were also obtained, including regions of pre-M/M protein. The presence of B cell epitopes in both mice and humans was demonstrated in the pre-M/M protein peptides. Peptides 3-31 and 103-124 elicited neutralizing antibodies against the four serotypes of Dengue virus. Virus-specific proliferative responses were demonstrated in mice immunized with non-conjugated peptides 3-31 and 57-92. Mice immunized with conjugated peptides 3-31, 57-92, and 69-93 were protected when they were challenged with Dengue-2 virus. Thus, the presence of sequential epitopes in Pre-M/M protein of Dengue-2 virus was demonstrated, as well as their relevance in the immune response against this flavivirus.
Inventors:
Vasques Lambudo, Susana
Guzman Tirad, Guadalupe
Gigien Nieto, Herald Enrique
Pardo Laso, Orlando Lewis
Cinea Santiago, Gly
Perez Dias, Anna Beatrice
Pupo Antunes, Marissa
Rodriguez Roche, Rosmari
Reyes Acosta, Oswald
Garai Perez, Iruda Elisa
Padron Palomares, Gabriel
Alvarez Bella, Myrin
Mollier Diaz, Lewis
Perez Insita, Omida
Pellegrino Martinez de la Coterra, Jose Luis
Guzman Tirad, Guadalupe
Gigien Nieto, Herald Enrique
Pardo Laso, Orlando Lewis
Cinea Santiago, Gly
Perez Dias, Anna Beatrice
Pupo Antunes, Marissa
Rodriguez Roche, Rosmari
Reyes Acosta, Oswald
Garai Perez, Iruda Elisa
Padron Palomares, Gabriel
Alvarez Bella, Myrin
Mollier Diaz, Lewis
Perez Insita, Omida
Pellegrino Martinez de la Coterra, Jose Luis
Application Number:
JP53348698A
Publication Date:
May 11, 2005
Filing Date:
January 13, 1998
Export Citation:
Assignee:
Centro de Heni Area Henetica I Biotechno Lohia (Seee Hebe)
Instituto de Medicina Tropical "Pedro Cori"
Instituto de Medicina Tropical "Pedro Cori"
International Classes:
C12N15/09; A61K38/00; A61K39/12; A61K39/395; A61P31/12; C07K14/18; C07K16/10; C12N15/40; A61K39/00; (IPC1-7): C07K14/18; A61K38/00; A61K39/395; A61P31/12; C07K16/10; C12N15/09
Other References:
Virology, 1991年,Vol.185, pp.505-8
The American Journal of Tropical Medicine and Hygiene, 1989年,Vol.41 (5), pp.576-80
Gene, 1989年,Vol.75, pp.197-211
Virology, 1986年,Vol.155, pp.77-88
Journal of General Virology, 1988年,Vol.69, pp.1391-8
The American Journal of Tropical Medicine and Hygiene, 1989年,Vol.41 (5), pp.576-80
Gene, 1989年,Vol.75, pp.197-211
Virology, 1986年,Vol.155, pp.77-88
Journal of General Virology, 1988年,Vol.69, pp.1391-8
Attorney, Agent or Firm:
Keiichiro Saikyo
Takeshi Sugiyama
Minetarou Hirose
Mikio Takeuchi
Takeshi Sugiyama
Minetarou Hirose
Mikio Takeuchi