PURPOSE: To produce a large amount of chitosan oligosaccharide useful in the field of pharmaceuticals, cosmetics, foods, etc., economically and efficiently, by partially hydrolyzing chitosan with hydrochloric acid, adding a methanol-contaning solvent to the hydrolyzed product, and dissolving and separating the objective material.
CONSTITUTION: Chitosan of formula is partially hydrolyzed with hydrochloric acid. 1pt. of the hydrolyzed product is added with usually 5W20pts.wt. of a solvent containing ≥60% (V/V) methanol (preferably ethanol, acetone, ether, etc. containing ≥90% methanol), and the mixture is stirred at ≤20°C for 2W3hr. The objective chitosan oligosaccharide can be produced by the dissolution and separation process taking advantage of the solubility difference.
NANJIYOU FUMIO
SAKAI KAZUO
