Title:
PREPARATION OF DEHYDROTESTOSTERONE
Document Type and Number:
Japanese Patent JPS5537121
Kind Code:
A
Abstract:
PURPOSE: To prepare 1,2-dehydrotestosterone (17β-hydroxy-androsta-1,4-dien-3- one; DHT) in large quantity, by culturing microorganisms capable of producing androsta-1,4-diene-3,17-dione (ADD), under controlled aeration.
CONSTITUTION: In the preparation of DHT from a sterole having a side chain at 17-position, using microorganisms capable of ADD, the aeration rate is controlled to >0.1VVM in the first 2/5 of the ADD fermentation period, and to ≤0.1VVM after an arbitrary time between 2/5 and 4/5 of the fermentation period. The microorganism of this invention is a mutant capable of producing ADD from steroles, or a strain capable of producing ADD in the presence of an oxidation inhibitor of a chelating agent.
Inventors:
IMADA YUKIO
KINOSHITA MASAYUKI
MACHIDA HAJIME
KINOSHITA MASAYUKI
MACHIDA HAJIME
Application Number:
JP10882078A
Publication Date:
March 15, 1980
Filing Date:
September 05, 1978
Export Citation:
Assignee:
MITSUBISHI CHEM IND
International Classes:
C12P33/18; C12P33/16; C12R1/01; C12R1/06; C12R1/13; C12R1/15; C12R1/32; C12R1/365; (IPC1-7): C12P33/16; C12R1/01; C12R1/06; C12R1/13; C12R1/15; C12R1/32; C12R1/365