Title:
PREPARATION OF ERYTHROPOIETIN-PRODUCING CELL
Document Type and Number:
Japanese Patent JPS62198388
Kind Code:
A
Abstract:
PURPOSE: To prepare an erythropoietin-producing cell, by introducing a mixed vector of an erythropoietin transducing vector and selected marker gene transduced vector into a mouse L929 cell using a specific technique.
CONSTITUTION: An aminoglycoside 3'-phosphotransferase gene (neo gene) derived from Tn which is a selected marker is inserted into the downstream of SV40 early gene of a shuttle vector pKSV-10 to prepare a selected marker neor gene transduced vector pKSVNeo, which is then mixed with an erythropoietin transducing vector pSVhEPX. The resultant mixed vector is further introduced into a mouse L929 cell by a DNA transfection technique using calcium phosphate to prepare the aimed erythropoietin-producing cell.
Inventors:
UEDA MASAJI
AKAI KUNIHISA
MURAKAMI MASAHIKO
CHIBA HIDEO
SASAKI RYUZO
AKAI KUNIHISA
MURAKAMI MASAHIKO
CHIBA HIDEO
SASAKI RYUZO
Application Number:
JP4227586A
Publication Date:
September 02, 1987
Filing Date:
February 27, 1986
Export Citation:
Assignee:
SNOW BRAND MILK PRODUCTS CO LTD
International Classes:
C12N15/09; C07K14/505; C12N5/00; C12N5/10; C12N15/00; C12N15/85; C12P21/00; C12P21/02; C12R1/91; (IPC1-7): C12N5/00; C12N15/00; C12P21/00; C12R1/91
Other References:
MOLECULAR BIOLOGICALS:CHEMICALS AND EQUIPMENT FOR MOLECULAR BIOLOGY=1985
JOURNAL OFMOLECULAR BIOLOGY=1981
JOURNAL OFMOLECULAR BIOLOGY=1981
Attorney, Agent or Firm:
Kiyoya Fujino (1 outside)
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