Title:
PREPARATION OF MATURE POLYPEPTIDE
Document Type and Number:
Japanese Patent JP3277200
Kind Code:
B2
Abstract:
PURPOSE: To simply obtain a mature polypeptide hardly contaminated by kydrolyzing a specific soluble precursor by a factor Xa immobilized on an insoluble solid support.
CONSTITUTION: A soluble polypeptide precursor of the formula; X-Ile-Glu-Gly- ArgY (X is Met or a peptide imparting solubility to the precursor; Ile- GluGly-Arg is a tetrapeptide recognized by factor Xa; Y is a mature peptide) is hydrolyzed by a factor Xa immobilized on an insoluble solid support.
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Inventors:
Guido Grandy
Giuliano Gari
Giuliano Gari
Application Number:
JP10018992A
Publication Date:
April 22, 2002
Filing Date:
March 27, 1992
Export Citation:
Assignee:
Biovitrum Activola Get
International Classes:
C07K14/61; C12N11/10; C12N15/62; C12P21/00; C12P21/02; C12P21/06; (IPC1-7): C12P21/06; C12N11/10; C12P21/00; C12P21/02
Domestic Patent References:
| JP22386A | ||||
| JP642583A | ||||
| JP1235598A |
Attorney, Agent or Firm:
Katsuzo Asakura
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