PURPOSE: To prepare a physiologically active substance in high yield, in a short time, by controlling the mixing ratio of adenosine-5'-mono-phosphate (AMP) and adenosine-5'-triphosphate (ATP) to a specific condition.
CONSTITUTION: (A) An enzyme (e.g. adenylic acid kinase) converting AMP to adenosine-5'-diphosphate (ADP) is made to react with an enzyme (e.g. acetic acid kinase) converting ADP to ATP to produce ATP from AMP, and (B) a physiologically active substance is synthesized by using the produced ATP. The AMP produced by reaction (B) and the ATP produced by the reaction (A) are mixed at a ratio defined by the formula [ATP and AMP are the concentration (mM); γ is the ratio of the activity of the immobilized enzyme to convert ADP to ATP to the activity of the immobilized enzyme to convert AMP to ADP, and is ≥1] to regenerate AMP to ATP, and the ATP is recycled to the step (B) for the synthesis of the physiologically active substance.
KONDOU HITOSHI
NAKAJIMA HIROSHI
IWASAKI TATSUO
RIKAGAKU KENKYUSHO
UNITIKA LTD
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