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Title:
PREPARATION OF PLACENTAL GONADOTROPIC HORMONE IN HIGH PURITY
Document Type and Number:
Japanese Patent JPS58157720
Kind Code:
A
Abstract:

PURPOSE: To obtain the titled hormone of high purity, by extracting a placental gonadotropic hormone of low purity with a neutralWweakly basic aqueous solution of a lower alcohol and a salt soluble therein, adding a lower alcohol thereto, and precipitating the titled hormone of high purity.

CONSTITUTION: A placental gonadotropic hormone of low purity is extracted with a neutralWweakly basic, preferably 7W8.5pH, aqueous solution of a lower alcohol, e.g. ethanol, and a salt soluble therein, e.g. ammonium acetate, and a lower alcohol is then added to the resultant extract to collect the formed precipitate, which is then dissolved in a weakly acidicWweakly basic, preferably 5.5W9pH, buffer solution, e.g. phosphoric acid buffer solution. The resultant solution is then brought into contact with a weakly basic anion exchanger, e.g. DEAE "Sephadex ", and the anion exchanger is then eluted with the buffer solution containing further a salt, e.g. common salt, to give the aimed placental gonadotropic hormone of high purity.


Inventors:
YUKI YOSHIKAZU
NISHIMURA TOYOHIKO
HIRATANI HAJIME
Application Number:
JP1982000041527
Publication Date:
September 19, 1983
Filing Date:
March 15, 1982
Export Citation:
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Assignee:
JAPAN CHEM RES
International Classes:
A61K35/22; A61K38/24; A61P5/00; C07K1/30; C07K14/435; C07K14/575; C07K14/59; C07K14/61; (IPC1-7): A61K35/22; A61K37/24