Title:
PREPARATION OF SECRETIN
Document Type and Number:
Japanese Patent JPS6069098
Kind Code:
A
Abstract:
Secretin, which cannot be prepared directly by genetic engineering because of its carboxylic acid carboxyl-terminus, can be obtained by preparing secretylglycine by genetic engineering and then obtaining secretin therefrom by enzymatic conversion of the terminal glycine radical. The gene for the secretylglycine is synthesized chemically from smaller single-stranded units which are linked enzymatically to give the complete gene, incorporated into a suitable vector and amplified therein, after which the peptide is isolated directly or as a fusion protein and, after cyanogen bromide cleavage, is converted enzymatically into secretin.
Inventors:
BUORUFUGANGU KEENIHI
YOOAHIMU ENGERUSU
OIGEEN UURUMAN
BUARUDEMARU BUETEKAMU
YOOAHIMU ENGERUSU
OIGEEN UURUMAN
BUARUDEMARU BUETEKAMU
Application Number:
JP16576184A
Publication Date:
April 19, 1985
Filing Date:
August 09, 1984
Export Citation:
Assignee:
HOECHST AG
International Classes:
A61K38/22; C07H21/04; C07K14/005; C07K14/195; C07K14/575; C07K14/645; C07K19/00; C12N15/09; C12N1/20; C12N15/00; C12N15/62; C12P21/02; C12R1/19; (IPC1-7): A61K37/24; C07H21/04; C07K15/04; C12N1/20; C12N15/00; C12P21/02; C12R1/19
Attorney, Agent or Firm:
Chika Takagi