PURPOSE: To efficiently produce a single chain urokinase by transforming animal cells with an expression vector containing a genome sequence coding urokinase followed by culturing the resultant transformant in a medium.
CONSTITUTION: First, an urokinase gene is recovered from a human genome library and transferred into an expression vector. Secondly, the resultant plasmid vector DNA is transferred into an animal cell to form a transformant capable of producing a coded protein. Subsequently, a glycosylated single-stranded prourokinase is recovered from the resulting transformant, and the objective single chain pro-urokinase is thus produced. The urokinase genome sequence is pref. a DNA fragment with about 3 Kb obtained by partial SmaI digestion of a genome DNA cloned into an appropriate cloning vector.
Nolli, Marialuisa
Robbiati, Federico Maria
Corti, Angelo
Blasi, Francesco
