Title:
ＤＮＡおよびＲＮＡの同時濃縮を含むプライマー伸長標的濃縮およびそれに対する向上
Document Type and Number:
Japanese Patent JP6970205
Kind Code:
B2
Abstract:
The present invention is a method and compositions for primer extension target enrichment of nucleic acids and improvements thereto including simultaneously enriching for RNA and DNA and optionally sequencing the enriched products. An embodiment of the present invention includes a method comprising the steps of: hybridizing a target-specific primer to a target DNA or RNA, wherein the primer comprises a target-binding region and a region of complementarity to an adaptor; extending the primer with a DNA polymerase or reverse transcriptase to form a primer extension product; contacting the product with an adaptor comprising a longer strand with a 5′-overhang having complementarity to said primer and a shorter strand comprising a universal priming site; hybridizing the adaptor to the product; and ligating one strand of the adaptor to the product to form a ligation product.
Inventors:
Godwin, Brian Christopher
Ozturku, Cedide
Ozturku, Cedide
Application Number:
JP2019548430A
Publication Date:
November 24, 2021
Filing Date:
March 07, 2018
Export Citation:
Assignee:
F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT
International Classes:
C12Q1/6806; C12Q1/6853; C12Q1/6855; C12Q1/686; C12Q1/6869; C12Q1/6876
Foreign References:
| WO2015121236A1 | ||||
| WO2017021449A1 |
Attorney, Agent or Firm:
Shinjiro Ono
Osamu Yamamoto
Toru Miyamae
Motoharu Nakanishi
Osamu Yamamoto
Toru Miyamae
Motoharu Nakanishi
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