Title:
PRODUCTION OF ASPURTEM
Document Type and Number:
Japanese Patent JPS56140897
Kind Code:
A
Abstract:
The artificial sweetener aspartame, a dipeptide with the formula Asp-Phe-me, is produced using a cloned micrcorganism. A DNA which codes for a large stable peptide comprised of the repeating amino acid sequence (Asp-Phe)n is inserted into a cloning vehicle which in turn is introduced into a suitable host microorganism. The host microorganism is cultured and the large peptide containing the repeating Asp-Phe sequence is harvested therefrom. The free carboxyl group of the large peptide is benzylated and then hydrolysed to benzyl Asp-Phe dipeptides. This dipeptide is methylated and then debenzylated to form aspartame.
Inventors:
CHIYANDERU PURAKASHIYU BOORU
JIYANAIN ESUTERO ROOZU
TOOMASU JIEEMUSU HOWAITO
JIYANAIN ESUTERO ROOZU
TOOMASU JIEEMUSU HOWAITO
Application Number:
JP3592681A
Publication Date:
November 04, 1981
Filing Date:
March 12, 1981
Export Citation:
Assignee:
CETUS CORP
International Classes:
C07H21/04; C07K1/113; C07K5/072; C07K5/075; C12N1/20; C12P21/02; C12N15/09; C12R1/125; C12R1/19; (IPC1-7): C07C103/52; C07H21/00; C12N1/20; C12N15/00; C12P19/34; C12P21/02; C12R1/125; C12R1/19
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