Title:
生物学的に活性な組換え神経栄養タンパク質の生産
Document Type and Number:
Japanese Patent JP3673278
Kind Code:
B2
Abstract:
A process for the production of biologically active recombinant neurotrophic factor from the NGF/BDNF family is described. The process is comprised of a) constructing a synthetic neurotrophic factor gene suitable for expression in a bacterial expression system; b) the synthetic neurotrophic factor gene is expressed in a bacterial expression system; c) the neurotrophic factor is solubilized and sulfonylated; d) sulfonylated neurotrophic factor is allowed to refold in the presence of polyethylene glycol and urea; and e) biologically active neurotrophic factor is isolated and purified.
Inventors:
Lyle, Jack
Kouno, Tadahiro
Bonham, Duane
Rozendahl, Mary S.
Kouno, Tadahiro
Bonham, Duane
Rozendahl, Mary S.
Application Number:
JP52904395A
Publication Date:
July 20, 2005
Filing Date:
May 02, 1995
Export Citation:
Assignee:
AMGEN INC.
International Classes:
C12N15/09; C07K1/113; C07K1/14; C07K14/475; C07K14/48; C07K14/50; C07K14/52; C12N1/20; C12N1/21; C12N15/00; C12N15/18; C12N15/70; C12P21/02; A61K38/00; (IPC1-7): C12N15/09; C07K14/48; C07K14/52; C12P21/02
Attorney, Agent or Firm:
Yusuke Hiraki
Ishii Teiji
Yasushi Hayakawa
Ishii Teiji
Yasushi Hayakawa
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