PURPOSE: To efficiently obtain deacetylcephalosporin C useful as an intermediate, etc., for synthesizing β-lactam antibiotics by reacting an enzyme source derived from a microorganism which belongs to Pseudomonas and capable of deacetylating cephalosporin C, etc., and then harvesting the reactional product from the reaction mixture.
CONSTITUTION: A microorganism which belongs to the Henus Pseudomonas [e.g. Pseudomonas sp.-14-2-2 strain (FERM P-3939)] is inoculated into a sterilized medium and subjected to shake culture at 26°C at 150rpm for 96hr and the cultured mixture is centrifuged and the bacterial cell is harvested to afford an enzyme source having activity capable of deacetylating cephalosporin C or 7-aminocephalosporanic acid. The enzyme source is added to a reactional liquid containing cephalosporin C or 7-aminocephalosporanic acid and subjected to shake culture reaction therewith at 26°C for 72hr to produce deacetylcephalosporin C or deacetyl-7-aminocephalosporanic acid in the reactional liquid and then the product is harvested from the reactional liquid to provide the objective deacetylcephalosporin C and its related compound.
OZAWA TATSUYA
WADA TADASHI
BIO LE KK
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