PRODUCTION OF GENETICALLY TRANSFORMED CUCUMBER CAPABLE OF MASSIVELY PRODUCING SUPEROXIDE DISMUTASE - Korea Institute of Science and Technology

Title:

PRODUCTION OF GENETICALLY TRANSFORMED CUCUMBER CAPABLE OF MASSIVELY PRODUCING SUPEROXIDE DISMUTASE

Document Type and Number:

Japanese Patent JP3307604

Kind Code:

B2

Abstract:

PROBLEM TO BE SOLVED: To obtain the subject plant by culturing the plant tissues together with a microorganism, genetically transformed by the aid of a vector containing a promoter predominantly expressing itself in a cucumber fruit, SOD gene and herbicide-resistant bar gene, and redifferentiating the cultured product.
SOLUTION: This method produces an expression vector for genetically transforming a plant, containing a gene promoter predominantly expressing itself in a cucumber fruit (e.g. ascorbate oxidase promoter), superoxide dismutase (SOD), e.g. the one isolated from cassava or the like, shown by the formula, and herbicide-resistant gene, and then genetically transforms agrobacteria by the aid of the expression vector, to produce a genetically transformed agrobacteria [e.g. Agrobacteria tumefaciens LBA4404 (KCTCO508BP)]. The genetically transformed agrobacteria is cultured together with plant tissues, e.g. those of cucumber, and redifferentiated, to obtain a genetically transformed plant, e.g. cucumber, capable of massively producing SOD, and resistant to herbicide and environmental stresses.

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Inventors:

Quark, Sun-Sue
Kim, J. Hoone
Lee, Hen-Soon
Kuon, Suku-Yoon

Application Number:

JP10593399A

Publication Date:

July 24, 2002

Filing Date:

April 13, 1999

Export Citation:

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Assignee:

Korea Institute of Science and Technology

International Classes:

A01H1/00; A01H4/00; A01H5/00; A23L1/30; A23L19/00; A61K8/00; A61K8/02; A61K8/66; A61K8/96; A61K8/97; A61K31/00; A61K36/42; A61K38/44; A61P17/00; C12N1/21; C12N5/10; C12N9/02; C12N15/09; C12N15/53; C12N15/82; A61K38/00; (IPC1-7): A01H1/00; A23L1/212; A23L1/30; A61K7/00; A61K35/78; A61K38/44; C12N1/21; C12N5/10; C12N9/02; C12N15/09

Domestic Patent References:

JP4501354A

Other References:

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ＰｌａｎＣｅｌｌＲｅｐ．，ｖｏｌ．１３［９］（１９９４），ｐ．４８１−４８８
ＰｌａｎｔＭｏｌ．Ｂｉｏｌ．，ｖｏｌ．２３（１９９３），ｐ．９１１−９１３

Attorney, Agent or Firm:

Takehiko Matsumoto

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