PURPOSE: To industrially advantageously obtain glucagon for medicine, etc., by eliminating an amino-protecting group by bonding amino group-protecting threonine to an insoluble resin bondable with carboxyl group, bonding amino acid in turn and eliminating peptide from the resin.
CONSTITUTION: A threonine residue in 29th position is introduced into an insoluble resin (e.g. benzhydrylamine resin) having a functional group bondable with a carboxyl group by bonding a carboxyl group of amino group-protecting threonine, a protecting group of the threonine residue is removed and resultant free amino group is subjected to peptide bonding with amino group-protecting amino acid in turn by according to an amino acid sequence of glucagon expressed by the formula to synthesize a peptide chain in extending each one by one from 28th positioned asparagine residue to first positioned histidine residue and resultant peptide is treated with anhydrous hydrogen fluoride to be separated from the resin, then the protecting group is removed to afford the objective glucagon useful for an action of increasing a blood sugar value or testing of growth hormone secreting function, etc.
HAMANAKA KAZUYA
NAGANO YUMIKO
TAKAHATA HIKARI
TOMIZAKI KINYA
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