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Title:
PRODUCTION OF HETEROLOGOUS HYDROGENASE IN BLUE-GREEN ALGAE, AND PRODUCTION OF HYDROGEN
Document Type and Number:
Japanese Patent JP3513560
Kind Code:
B2
Abstract:

PROBLEM TO BE SOLVED: To obtain a hydrogenase capable of producing hydrogen during anaerobic incubation of a transformed blue-green algae in which a hydrogenase gene was introduced, by culturing a specific blue-green algae and forming and accumulating the hydrogenase in the cells.
SOLUTION: This hydrogenase is obtained by culturing a blue-green algae, for example, belonging to the genus Synechococcus in which a recombinant vector was introduced which has an exogenous hydrogenase gene having Shine- Dalgarno sequence consisting of AAGGAA upstream a hydrogenase structural gene from a microorganism such as Clostridium pasteurianum followed by forming and accumulating hydrogenase in th cell. It, is preferable to produce the hydrogen by culturing the blue-green algae, into which the recombinant, vector was introduced. under the light.


Inventors:
Koike, Hiromitsu
Aoyama, Katsuhiro
Asada, Yasuo
Miyake, Atsushi
Miyake, Masato
Joerg, Schnakenberg
Application Number:
JP1998000062601
Publication Date:
January 23, 2004
Filing Date:
March 13, 1998
Export Citation:
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Assignee:
AGENCY OF IND SCIENCE & TECHNOL
RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTH
International Classes:
C12N15/09; C12N1/12; C12N1/13; C12N9/02; C12P3/00; (IPC1-7): C12N15/09; C12N1/12; C12N9/02; C12P3/00