To provide a silk fibroin film usable as a medical material and the like and having flexibility and adhesion.

The silk fibroin film which is produced by dissolving the silk fibroin with high-concentration neutral salt solution such as calcium chloride, removing the neutral salt therefrom by dialysis, and casting it on a glass plate, is treated with steam so as to accelerate crystallization of the silk fibroin and provide the film with the flexibility.