PURPOSE: To easily obtain the titled compound useful as a remedy for diabetes, etc., in high purity and yield, by carrying out fractional crystallization of a mixture of specific glucosylmoranoline, oligoglucosylmoranoline and moranoline using methanol as a crystallization solvent.
CONSTITUTION: An aqueous solution containing moranoline of formula I and cyclodextrin or soluble starch is successively treated with cyclodextrin glucosyl transferase and glucoamylase. The obtained mixture of glucosylmoranoline of formula II, oligoglucosylmoranoline of formula III (n is 1W5) and the compound of formula I is dissolved in water and the obtained solution is added with methanol. The mixture is stirred preferably at an agitation rate of about 200rpm for 24hr to effect the crystallization and obtain the objective compound which is a molecular compound of methanol and the compound of formula II. Methanol can be removed from the molecular compound by the fractional crystallization using ethanol as the crystallization solvent.
YAMAMOTO MASASHI
MARUO SHIGEAKI
SUGIYAMA MAKOTO
JPS61115093A | 1986-06-02 |
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