Title:
C4bpスカフォールドを使用した、多量体融合タンパク質の産生
Document Type and Number:
Japanese Patent JP2005535353
Kind Code:
A
Abstract:
The present invention provides a method for obtaining a recombinant fusion protein comprising a scaffold of a C-terminal core protein of C4bp alpha chain, said recombinant fusion protein being capable of forming multimers in soluble form in a prokaryotic host cell, the method including the steps of (i) providing a prokaryotic host cell carrying a nucleicacid encoding said recombinant protein operably linked to a promoter functional in said prokaryotic cell; (ii) culturing the host cell under conditions wherein said recombinant protein is expressed; and (iii) recovering the recombinant protein wherein said protein is recovered in multimeric form without performing a scaffold refolding step.
Inventors:
Garnier, Lawrence
Hill, fergal
Julian, Michelle
Hill, fergal
Julian, Michelle
Application Number:
JP2004532084A
Publication Date:
November 24, 2005
Filing Date:
August 12, 2003
Export Citation:
Assignee:
Avidis S
International Classes:
C12N15/09; A61K35/76; A61K38/00; A61K38/17; A61K48/00; A61P37/02; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/62; C12N15/63; C12P21/02; (IPC1-7): C12N15/09; A61K35/76; A61K38/00; A61K48/00; A61P37/02; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12P21/02
Attorney, Agent or Firm:
Yusuke Hiraki
Ishii Teiji
Satoshi Fujita
Ishii Teiji
Satoshi Fujita