PURPOSE: To easily obtain the titled enzyme on an industrial scale, by transforming a specific microorganism e.g. with a chromosome DNA fragment containing an N-acylneuraminic aldolase (N-A) gene and culturing the transformed microorganism.
CONSTITUTION: A mutant (A) such as Escherichia coli M8328 is produced by a mutagenic treatment of e.g. Escherichia coli belonging to Escherichia genus. An N-A producing strain of Escherichia genus is treated by phenol, etc., to obtain a chromosome DNA, which is cut with a restriction enzyme, etc. The obtained chromosome DNA fragment is inserted and linked to a vector DNA [e.g. strain of ColEI] to obtain a recombinant plasmid (B). The component B is introduced into the strain A to form a transformed strain (C). The strain C is cultured in a liquid medium containing a carbon source, a nitrogen source, etc., and free from sialic acid at 20W45°C and 6W9pH for 10W50hr under aeration and agitation. N-A produced and accumulated in the cell of the strain C is collected from the cell.
| WO/2002/055651 | PROCESSES AND VECTORS FOR PLASTID TRANSFORMATION |
| JP4659236 | How to measure cytotoxic activity |
| WO/1998/015633 | $i(CHRYSOSPORIUM) CELLULASE AND METHODS OF USE |
TSUKADA YOJI
OOTA YASUHIRO
KIMURA HIKARI
