PURPOSE: To obtain N-carbamoyl-D-phenylglycine in high efficiency by making microorganisms act a on 5-phenylhydantoin at separate two stages of reaction pHs 6.0-8.5 and 7.0-9.0.
CONSTITUTION: At the first stage, bacteria or immobilized ones of microorganisms such as Pseudomonas is made to act on a suspension of a 5-phenylhydantoin (5-PeH) of general formula I. The pH during the reaction is retained at 6.0-8.5 by dripping an aqueous alkali solution such as of sodium hydroxide. Thence, the resultant reaction liquid is put to solid/liquid separation to obtain a clear reaction solution. Next, at the second stage, the the pH of this clear solution is retained at 7.0-9.0, and the bacteria or immobilized ones are newly made to act on the system. The resultant reaction solution is condensed and the pH is brought to the isoelectric point(2), thus obtaining the objective N-carbamoyl-D-phenylglycine (CDPG) of general formula II. Through the above-mentioned processes, the CDPG can be industrially advantageously obtained in high yield at low cost.
TAKEICHI MAMORU
TAWAKI SHINICHIROU
AIKAWA TOSHIKAZU
| JPS5586A | 1980-01-05 | |||
| JPS5455788A | 1979-05-04 |
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