Title:
PRODUCTION OF NON-NATURAL CEPHALOSPORINE
Document Type and Number:
Japanese Patent JPS5991895
Kind Code:
A
Abstract:
The enzymes, cyclase, epimerase and a ring expansion enzyme, are isolated separately from a cell-free extract of a prokaryotic beta-lactam producing organism. The isolated enzymes are used to produce unnatural cephalosporins from polypeptide precursors. Isolation is carried out by adding ammonium sulfate to 40% saturation to the cell-free extract to precipitate contaminating proteins, adding ammonium sulfate to 70% saturation to the resultant supernatant to precipitate the enzymes, suspending the precipitated enzymes in a buffer, separating epimerase from the suspension by gel filtration, and separating cyclase and the ring expansion enzyme from each other by ion exchange chromatography.
Inventors:
SAURU UORUFU
DONARUDO UESUTOREEKU
SUUZAN JIENSEN
DONARUDO UESUTOREEKU
SUUZAN JIENSEN
Application Number:
JP15174783A
Publication Date:
May 26, 1984
Filing Date:
August 22, 1983
Export Citation:
Assignee:
UNIV KINGSTON
International Classes:
C12N11/08; C12N9/00; C12N9/90; C12N11/18; C12P35/00; C12P35/06; C12P37/00; (IPC1-7): C12N11/08; C12P35/00
Attorney, Agent or Firm:
Aoki Akira