Title:
ポリオールからのポリヒドロキシアルカノエートの産生
Document Type and Number:
Japanese Patent JP5042407
Kind Code:
B2
Abstract:
Organisms arc provided which express enzymes such as glycerol dehydratase, diol dehydratase, acyl-CoA transferase, acyl-CoA synthetase ²-ketothiolase, a;xtoaeetyl-CoA reductase, PHA synthase, glycerol-3-phosphate dehydrogenase and glycerol-3-pbospharase, which are useful for the production of PHAs. In some cases one or more of these genes are native to the host organism and the remainder are provided from transgenes. These organisms produce poJy(3-hydroxyalkanoare) homopolymers or co-polymers incorporating 3-hydroxypropionate or 3-hydroxyvalerate monomers wherein the 3-hydroxynropionate and 3-hydroxyvalerate units are derived from the enzyme catalysed conversion of diols. Suitable diols that can be used include 1,2-propanediol, 1,3-propanediol and glycerol. Biochemical pathways for obtaining the glycerol from normal cellular metabolites are also described. The PHA polymers are readily recovered and industrially useful as polymers or as starting materials for a range of chemical intermediates including 1,3-propanediol, 3-hydroxypropionaldehyde, -acrylics, malonic acid, esters and amines.
Inventors:
Sclery, frank
Peoples, Oliver Pea.
Peoples, Oliver Pea.
Application Number:
JP2000563821A
Publication Date:
October 03, 2012
Filing Date:
August 04, 1999
Export Citation:
Assignee:
Metabolics, Incorporated
International Classes:
A01H1/00; C12N15/09; C08G8/04; C08G8/26; C12N1/21; C12N15/52; C12N15/82; C12P7/42; C12P7/62; C12R1/19
Domestic Patent References:
| JP2109985A |
Foreign References:
| WO1998004713A1 | ||||
| WO1991000917A1 |
Other References:
The Journal of Biological Chemistry,1998年,Vol.273, No.37,p.24044-24051
Biotechnology and Bioengineering,1996年,Vol.49,p.495-503
Applied and Environmental Microbiology,1998年 1月,Vol.64, No.1,p.98-105
Journal of Bacteriology,1995年,Vol.177, No.8,p.2151-2156
Journal of Bacteriology,1990年,Vol.172, No.11,p.6557-6567
Biotechnology and Bioengineering,1996年,Vol.49,p.495-503
Applied and Environmental Microbiology,1998年 1月,Vol.64, No.1,p.98-105
Journal of Bacteriology,1995年,Vol.177, No.8,p.2151-2156
Journal of Bacteriology,1990年,Vol.172, No.11,p.6557-6567
Attorney, Agent or Firm:
Yoshio Kawaguchi
Makoto Ono
Chihiro Watanabe
Kenkyo Kanayama
Katsuma Osaki
Mitsuaki Tsubokura
Makoto Ono
Chihiro Watanabe
Kenkyo Kanayama
Katsuma Osaki
Mitsuaki Tsubokura