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Title:
PRODUCTION OF PORCINE EPIDEMIC DIARRHEA VIRUS (PEDV) SENSITIVE CELL
Document Type and Number:
Japanese Patent JP10084951
Kind Code:
A
Abstract:

To produce porcine epidemic diarrhea virus(PEDV) producing cells effectively, useful for an inactivated virus vaccine, a diagnosis, etc., by culturing cultured cells originated from a pig with a trypsin-added cell culturing liquid and collecting trypsin resistant cells.

This method for producing PEDV producing cells, is to culture cultured cells originated from a pig with a trypsin-added culturing solution obtained by mixing 10vol.% tryptose phosphate broth, 100μg/ml kanamycin, 1μg/ml fungizone and 10μg/ml trypsin with an Eagle MEM solution, and collect trypsin resistant cells. Further, it is preferable to inoculate PEDV to PEDV producing trypsin resistant renal cells (TR-SK cells) or PEDV producing trypsin resistant porcine intestine epithelial cells (TR-CCI cells), culture them with a culturing liquid added with 75-100μg/ml trypsin for growing the PEDV, and then isolate the virus.


Inventors:
Nishimura, Masaaki
Application Number:
JP1996000245807
Publication Date:
April 07, 1998
Filing Date:
September 18, 1996
Export Citation:
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Assignee:
NATL FEDELATION OF AGRICULT COOP ASSOC
International Classes:
G01N33/53; C12N5/07; C12N5/071; C12N7/00; G01N33/569; G01N33/53; C12N5/07; C12N5/071; C12N7/00; G01N33/569; (IPC1-7): C12N5/06; C12N7/00; G01N33/53; G01N33/569