PURPOSE: To synthesize a saccharide mono-fatty acid ester in a short time in high synthetic attainment ratio by blending a specific fatty acid with a spe cific saccharide such as monosaccharide and carrying out enzymatic reaction using thermostable immobilized lipase in the presence of an organic solvent.
CONSTITUTION: 6-22C saturated fatty acid such as caproic acid, an unsaturated fatty acid or an ester such as methyl caproate of the fatty acid and 1-3C lower alcohol such as methanol is blended with a saccharide selected from 5-7C monosaccharide such as arabinose not containing a substituent group, disaccharide comprising a hexose such as aldose and 4-6C sugaralcohol such as erythritol and enzymatic reaction is carried out using thermostable lipase derived from immobilized Candida antarctica in the presence of an organic solvent such as benzene. Then, after the reaction is over, a saccharide mono- ratty acid ester is separated and collected from the reaction product by a conven tional procedure. Consequently, the saccharide mono-fatty acid ester is separated and collected from the reaction product can be synthesized in high synthetic attainment ratio, in high content, industrially and advantageously.
KANDA MIDORI
TODA HARUHIKO
KITANO KYOZO