Title:
PRODUCTION OF TRANSGLUTAMINASE DERIVED FROM MICROORGANISM BELONGING TO GENUS BACILLUS
Document Type and Number:
Japanese Patent JP3873408
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To provide a method for producing a transglutaminase derived from Bacillus subtilis in an active form by using a microbe belonging to the genus Escherichia.
SOLUTION: A microbe belonging to the genus Escherichia having a DNA fragment of a transglutaminase gene derived from the microbe belonging to the genus Bacillus is cultivated, and the expression of the transglutaminase gene is induced in the period from the time deteriorating the logarithmic propagation of the microbes to the time reaching stationary phase of the growth of the microbes. The transglutaminase is accumulated in the microbes belonging to the genus Escherichia.
Inventors:
Kenichi Hashiguchi
Kenzo Yokozeki
Kenzo Yokozeki
Application Number:
JP30615597A
Publication Date:
January 24, 2007
Filing Date:
November 07, 1997
Export Citation:
Assignee:
AJINOMOTO CO.,LTD.
International Classes:
C12N15/09; C12N9/10; C12N15/00; C12R1/125; C12R1/19; (IPC1-7): C12N15/09; C12N9/10; //(C12N15/09; C12R1:125); (C12N9/10; C12R1:19)
Domestic Patent References:
| JP9131180A | ||||
| JP1100200A | ||||
| JP6228194A |
Other References:
Biotechnol. Lett.,1997年 8月,19 [8],p.735-739
J. Microbiol. Biotechnol.,1991年,1 [4],p.274-280
J. Microbiol. Biotechnol.,1991年,1 [4],p.274-280
Attorney, Agent or Firm:
Tsutomu Toyama
Hidemi Matsukura
Yoshiyuki Kawaguchi
Hidemi Matsukura
Yoshiyuki Kawaguchi