Title:
PRODULCTION METHOD FOR ANGELICA ACUTILOBA BY ITS ORGAN CULTURE
Document Type and Number:
Japanese Patent JP2561202
Kind Code:
B2
Abstract:
PURPOSE: To provide the subject production method so designed that the organs of Angelica acutiloba for which efficient proliferation method for varieties of superior quality has not been established are sterilely cultured to enable mass clone proliferation and also plant body regeneration from cells.
CONSTITUTION: The organs of Angelica acutiloba are cultured in an artificial medium with the nitrogen level adjusted to ≤20 mM to induce and proliferate adventitious seedlings or callus. Thence, the resulting seedlings or callus is transplanted to an artificial medium with the nitrogen level adjusted to ≥20 mM to effect regeneration into a plant body. Thus, for Angelica acutiloba useful as a crude drug, it has been made possible to perform efficient mass clone proliferation of varieties of superior quality. Also, plant body regeneration from cells has become possible, facilitating breeding.
Inventors:
MURAKAMI AKIRA
TAKASHIMA YASUKO
YOSHIOKA HIDETOSHI
MURAKAMI KUNIMUTSU
TAKASHIMA YASUKO
YOSHIOKA HIDETOSHI
MURAKAMI KUNIMUTSU
Application Number:
JP15734992A
Publication Date:
December 04, 1996
Filing Date:
May 26, 1992
Export Citation:
Assignee:
NIPPON SEISHI KK
International Classes:
A01H4/00; C12N5/04; (IPC1-7): A01H4/00
Other References:
REP KYUSHU BR CROP SCI SOC=1989
Attorney, Agent or Firm:
Kiyoshi Minoura