PROLACTIN POLYPEPTIDE IN FISH

Japanese Patent JPS6287600

A

NEW MATERIAL:A compound having the peptide sequence of the formula.

USE: A growth promotor for fish.

PREPARATION: For example, hypophysis is extracted from white salmons, frozen, crushed for solubilization, and ultracentrifuged to collect RNA as a precipitate. The RNA is dissolved in a high-concentration salt solution of NaCl or the like, and passed through an oligo(dT)cellulose column to obtained poly(A)RNA. The product is allowed to bond to a suitable vector primer, a reverse transcriptase is allowed to act on to prepare cDNA, which is cyclized by reaction in the presence of a suitable linker DNA and an enzyme. Further, the cyclized DNA is allowed to react with a nucleoside in the presence of an enzyme to form a recombined plasmid containing double-stranded cDNA. Then, the cDNA is introduced into Escherishia coil to effect transformation. Finally the microorganism is cultured to obtain prolactin polypeptide in fish.

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KUWANA YOSHIHISA
SATO MORIYUKI
ITOU SEIGA

JP22753185A

April 22, 1987

October 12, 1985

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KYOWA HAKKO KOGYO KK

C12N15/09; A01K61/00; C07K14/00; C07K14/575; C12N1/00; C12N1/20; C12N15/70; C12P21/02; C12R1/19; (IPC1-7): A01K61/00; A23K1/165; C07K13/00; C12N1/00; C12N1/20; C12N15/00; C12P21/02; C12R1/19

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