Title:
細胞透過のための過剰に荷電されたタンパク質
Document Type and Number:
Japanese Patent JP2012525146
Kind Code:
A
Abstract:
Compositions, preparations, systems, and related methods for delivering a supercharged protein, or a complex of a supercharged protein and an agent (e.g., nucleic acids, peptides, proteins, small molecules) to cells are provided. Such systems and methods include the use of supercharged proteins. For example, superpositively charged proteins may be associated with nucleic acids (which typically have a net negative charge) via electrostatic interactions. In some embodiments, such systems and methods involve altering the primary sequence of a protein in order to “supercharge” the protein (e.g., to generate a superpositively-charged protein). In some embodiments, complexes comprising supercharged proteins and one or more agents to be delivered are useful as therapeutic agents. In some embodiments, complexes and/or pharmaceutical compositions thereof are administered to a subject in need thereof. The inventive complexes or pharmaceutical compositions thereof may be used to treat proliferative diseases, infectious diseases, cardiovascular diseases, inborn errors in metabolism, genetic diseases, etc.
Inventors:
Ryu, David Earl.
McNorton, Brian Earl.
Chronican, James Joseph
Thompson, David B.
McNorton, Brian Earl.
Chronican, James Joseph
Thompson, David B.
Application Number:
JP2012508478A
Publication Date:
October 22, 2012
Filing Date:
April 28, 2010
Export Citation:
Assignee:
President and Fellows of Harvard College
International Classes:
C12N15/09; A61K31/7105; A61K38/00; A61K47/42; A61K47/48; A61K48/00; C07K14/01; C07K14/435; C07K14/475; C07K19/00; C12N9/00; C12N9/22; C12N9/50; C12N15/113; C12Q1/02
Domestic Patent References:
| JP2011507588A | 2011-03-10 |
Foreign References:
| US20070105182A1 | 2007-05-10 |
Other References:
PROC.NATL.ACAD.SCI.USA,2009,106(15),P.6111-6, JPN6014040069
GENESIS,2007,45(8),P.508-17, JPN6014040071
CELL STEM CELL,2009,4(5),P.381-4, EPUB 2009 APR 23, JPN6014040073
GENESIS,2007,45(8),P.508-17, JPN6014040071
CELL STEM CELL,2009,4(5),P.381-4, EPUB 2009 APR 23, JPN6014040073
Attorney, Agent or Firm:
Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita
Takaaki Yasumura
Natsuki Morishita