Title:
PURIFICATION OF SYNTHETIC DNA
Document Type and Number:
Japanese Patent JPH04149188
Kind Code:
A
Abstract:
PURPOSE: To simplify purification of synthetic DNA by taking out a synthetic DNA from a carrier, eliminating a protecting group followed by using gel filtration method.
CONSTITUTION: A synthetic DNA taken out from a carrier and deprotected is purified by using a disposable gel filtration type column, fractions obtained by collecting dividedly several times by passing sterilized water through the column are confirmed by measuring absorption spectrum at 300-220nm to purify the objective synthetic DNA.
Inventors:
SHIMIZU KUREYOSHI
Application Number:
JP27436390A
Publication Date:
May 22, 1992
Filing Date:
October 12, 1990
Export Citation:
Assignee:
SHIMADZU CORP
International Classes:
C07H1/06; C07H21/04; (IPC1-7): C07H1/06; C07H21/04
Attorney, Agent or Firm:
Toshifumi Kita (1 person outside)