Title:
PURIFICATION OF THROMBIN SUBSTRATE OR INHIBITOR PURIFIED BY MONOCLONAL ANTIBODY
Document Type and Number:
Japanese Patent JP2000143692
Kind Code:
A
Abstract:
To provide the subject purification method comprising easily removing monoclonal antibody and/or virus left in a thrombin substrate or inhibitor afforded by purification using monoclonal antibody by making use of a new and inexpensive separation/purification means with a simple operation and in a short man-hour.
This purification method comprises purifying one thrombin substrate or inhibitor purified using a monoclonal antibody by affinity chromatography using an affinity adsorbent bearing, as ligand, thrombin or a thrombin-like substance freed from nucleophilicity (deactivated) without causing steric hindrance in its binding to the substrate and/or inhibitor.
Inventors:
SUZUKI TOYOAKI
HOSOKAWA KAZUYA
NAGATA MASANORI
HOSOKAWA KAZUYA
NAGATA MASANORI
Application Number:
JP32104298A
Publication Date:
May 26, 2000
Filing Date:
November 11, 1998
Export Citation:
Assignee:
FUJIMORI KOGYO CO
International Classes:
C12N15/09; A61K35/14; C07K1/22; C07K14/705; C07K14/745; C07K14/75; C12N9/50; C12N9/64; C12N9/74; C12N9/99; (IPC1-7): C07K1/22; A61K35/14; C07K14/705; C07K14/745; C07K14/75; C12N9/50; C12N9/64; C12N9/74; C12N9/99; C12N15/09
Attorney, Agent or Firm:
Mikio Hatta (3 outside)
Previous Patent: JP2000143691
Next Patent: ACTIVATING FACTOR FOR PLATELET-DERIVED LEUKOCYTE PHAGOCYSTOSIS STHENIC FACTOR
Next Patent: ACTIVATING FACTOR FOR PLATELET-DERIVED LEUKOCYTE PHAGOCYSTOSIS STHENIC FACTOR