Title:
核酸を定量するための定量化スキーム
Document Type and Number:
Japanese Patent JP2007506418
Kind Code:
A
Abstract:
Methods, apparatus, and systems, including computer program products, implement techniques for determining an amount of target nucleic acid ("target") in a sample. Signal data is received for a plurality of cycles of an amplification experiment performed on the target and a standard nucleic acid ("standard"). The signal data includes a series of signal values indicating a quantity of standard present during cycles of the standard amplification, and a series of signal values indicating a quantity of target present during cycles of the target amplification. A target growth curve value is defined using the target signal values and a standard growth curve value is defined using the standard signal values. An initial amount of the target is calculated according to a calibration equation using an initial amount of the standard, and the target and standard growth curve values, where the calibration equation is a nonlinear equation.
Inventors:
Zühler, Gunther
Baby, liner
Franken, Norbert
Riding, hermann
Pinzle-Obel, Eudit
Beth, Thomas
Baby, liner
Franken, Norbert
Riding, hermann
Pinzle-Obel, Eudit
Beth, Thomas
Application Number:
JP2006527357A
Publication Date:
March 22, 2007
Filing Date:
September 24, 2004
Export Citation:
Assignee:
F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT
International Classes:
C12Q1/68; C12M1/34; G01N33/50; C12N15/09; C12P19/34; G01N21/78; G01N33/48; G01N33/53; G01N33/536; G01N33/566; G06F19/00
Attorney, Agent or Firm:
Yoshinori Hosoda