Title:
ライブラリーの定量および定性
Document Type and Number:
Japanese Patent JP6983906
Kind Code:
B2
Abstract:
Described herein are methods, compositions, and kits for library quantitation and qualification. Some embodiments relate to a method of library quantitation. For example, the method may include providing DNA fragments, amplifying the DNA fragments by polymerase chain reaction (PCR) in the presence of primers each labeled with a fluorophore. In these instances, only a predetermined number of fluorophores are attached to each DNA fragment. The method may further include detecting a fluorescent signal produced by the amplified DNA fragments and calculating a number of the amplified DNA fragments based on the detected fluorescent signal.
Inventors:
Ammores, Douglas A.
Lee, Bin
Schroeder, Benjamin Gee.
Fequete, Richard A.
Lee, Bin
Schroeder, Benjamin Gee.
Fequete, Richard A.
Application Number:
JP2019555479A
Publication Date:
December 17, 2021
Filing Date:
April 11, 2017
Export Citation:
Assignee:
Nugen Technologies, Inc.
International Classes:
C12Q1/6851; C12Q1/6855; C12Q1/686; C12Q1/6869; C40B40/06; G16B25/20; G16B40/10
Domestic Patent References:
| JP2015511819A | ||||
| JP2013111081A |
Foreign References:
| US20140186827 | ||||
| US20100069250 | ||||
| US20130231253 | ||||
| US20020197611 | ||||
| CN105890722A |
Attorney, Agent or Firm:
Hidesaku Yamamoto
Natsuki Morishita
Natsuki Morishita