QUICK AND SIMPLE DETECTING METHOD FOR UROPHANIC DISEASE MARKER PROTEIN USING LIGHT-ABSORBING LABEL

Japanese Patent JP3674529

B2

PROBLEM TO BE SOLVED: To provide a method and a reagent for detecting a trace amount of protein, capable of easily and quickly detecting and/or measuring basic protein of an urophanic disease marker, such as the Bence-Jones protein(BJP) useful as a diagnostic marker for multiple myeloma with high sensitivity.
SOLUTION: This method and its measurement reagent set includes a process for extracting and separating the BJP inside a urine sample from other proteins in urine by aqueous two phase partition using water soluble polymer and cation modified polymer, a process cleaning an extracted phase, containing the BJP of an objective protein, and a process reacting the extracted BJP with a pigment. Coloration of a BJP-pigment combination can be qualitatively and/or quantitatively analyzed and evaluated by a sensitizing function under coexistence of the water soluble polymer. The basic protein, belonging to prerenal protein with a high isoelectric point such as the disease diagnostic marker BJP, can be detected and measured easily and quickly with high sensitivity by solid phase extraction/pigment bonding.

JP2002533722	[Title of the Invention] A biological sampling device and a collecting method in a remote location
WO/1995/018373	URINE ANALYZING UNIT OF TYPE MOUNTED ON CLOSET
WO/2002/031495	ELECTRON PARAMAGNETIC RESONANCE METHOD FOR DIAGNOSIS OF ACTIVE NEPHRITIS

Emiko Kaneko
Takashi Kurasawa
Takao Yotsuyanagi
Kunihiko Akatsuka
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JP2001105742A

July 20, 2005

April 04, 2001

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Tohoku Techno Arch Co., Ltd.

G01N33/493; G01N21/78; G01N33/483; G01N33/50; G01N33/52; G01N33/58; G01N33/68; (IPC1-7): G01N33/52; G01N21/78; G01N33/68

JP2167475A
JP7098318A

Kazuo Shigenobu
Mizuno Akinori
Hideo Shimizu

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