Title:
QUINOPROTEIN GLUCOSE DEHYDROGENASE, AND METHOD FOR PRODUCING THE SAME
Document Type and Number:
Japanese Patent JP2011083251
Kind Code:
A
Abstract:
To provide a quinoprotein glucose dehydrogenase excellent in heat resistance and pH resistance, and also excellent in temperature stability and pH stability.
The quinoprotein glucose dehydrogenase, which is derived from Pyrobaculum, includes the following properties: (1) the molecular weight (SDS-PAGE method) is 33±6 kDa; (2) a monomer protein; (3) the optimum pH is 7-9; (4) the optimum temperature is 75°C or higher; (5) the temperature stability is 80°C or lower; and (6) the pH stability is 4-11. A method for producing the enzyme, a conjugate of the enzyme and a coenzyme, a glucose measurement kit, a gene encoding the enzyme, a vector including the gene, and a transformant including the vector, are also provided.
Inventors:
SAKURABA HARUHIKO
YOKONO KAORI
YOKONO KAORI
Application Number:
JP2009239902A
Publication Date:
April 28, 2011
Filing Date:
October 16, 2009
Export Citation:
Assignee:
UNIV KAGAWA
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/04; C12Q1/32
Domestic Patent References:
| JP2006238734A | 2006-09-14 | |||
| JP2006141263A | 2006-06-08 |
Foreign References:
| WO2007116710A1 | 2007-10-18 |
Other References:
JPN6013054348; Ｂｉｏｃｈｉｍ． Ｂｉｏｐｈｙｓ． Ａｃｔａ． Ｖｏｌ． １６４７, 2003, ｐ． １４３-１５１
JPN6013054349; ＮＣＢＩ Ｒｅｆｅｒｅｎｃｅ Ｓｅｑｕｅｎｃｅ， ［ｏｎｌｉｎｅ］， Ａｃｃｅｓｓｉｏｎ Ｎｏ． ＮＰ＿５６０１８６， ＜ｈｔｔｐ：／／ｗｗｗ．ｎｃｂｉ．ｎｌｍ．ｎｉｈ．ｇｏｖ／ｐｒｏｔｅｉｎ／１８３ , 20090424
JPN6014004959; Ｊ． Ｂｉｏｌ． Ｉｎｏｒｇ． Ｃｈｅｍ． Ｖｏｌ． ５, 2000, ｐ． ４０２-４０８
JPN6014004961; Ｊ． Ｂａｃｔｅｒｉｏｌ． Ｖｏｌ． １８３， Ｎｏ． １９, 2001, ｐ． ５４９１-５４９５
JPN6014004964; ＦＥＢＳ Ｌｅｔｔ． Ｖｏｌ． ４６０, 1999, ｐ． ５０５-５１２
JPN6013054349; ＮＣＢＩ Ｒｅｆｅｒｅｎｃｅ Ｓｅｑｕｅｎｃｅ， ［ｏｎｌｉｎｅ］， Ａｃｃｅｓｓｉｏｎ Ｎｏ． ＮＰ＿５６０１８６， ＜ｈｔｔｐ：／／ｗｗｗ．ｎｃｂｉ．ｎｌｍ．ｎｉｈ．ｇｏｖ／ｐｒｏｔｅｉｎ／１８３ , 20090424
JPN6014004959; Ｊ． Ｂｉｏｌ． Ｉｎｏｒｇ． Ｃｈｅｍ． Ｖｏｌ． ５, 2000, ｐ． ４０２-４０８
JPN6014004961; Ｊ． Ｂａｃｔｅｒｉｏｌ． Ｖｏｌ． １８３， Ｎｏ． １９, 2001, ｐ． ５４９１-５４９５
JPN6014004964; ＦＥＢＳ Ｌｅｔｔ． Ｖｏｌ． ４６０, 1999, ｐ． ５０５-５１２
Attorney, Agent or Firm:
Koichiro Tsujimaru
Yumi Nakayama
Reiko Yoshida
Isaji So
Yumi Nakayama
Reiko Yoshida
Isaji So