ＲＮＡ増幅法およびＨＩＶ−１感染診断方法

Japanese Patent JP6593984

B2

PROBLEM TO BE SOLVED: To provide an RNA amplification method which can stably detect HIV-1 in which genome mutation occurs easily, at a temperature condition of 41°C or less where it is easy to heat.SOLUTION: As a result of the detail analysis of the mutation reported in HIV-1, we found out that in an HIV-1 genome, an RNase H genome region has less mutation than other regions have. Thereby, RNA is amplified using two kinds of DNA primers which can specifically bind to both ends of the amplification gene moiety of a target gene, a reverse transcriptase, and an RNA polymerase, with all or a part of human immunodeficiency virus type I (HIV-1) RNase H DNA as a target.SELECTED DRAWING: Figure 1

JP2004081219	HIV-I POLYNUCLEOTIDE
JP3428012	INDUSTRIAL APPLICABILITY The nucleotide sequences from the genomes of HIV-1, HIV-2 and SIV type retroviruses, and in particular for the amplification of the genomes of these retroviruses and "in vitro" of infection by these viruses. Application of these sequences for diagnosis
WO/1997/046687	VECTORS COMPRISING SAR ELEMENTS

Kiyoshi Hokawa
Naoya Ichimura
Takakiyo Tada
Takaaki Hirano

JP2014195968A

October 23, 2019

September 26, 2014

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Kri Co., Ltd.

C12N15/49; C12Q1/6844; C12Q1/6888

JP2004500014A
JP2002320481A
JP2014082936A

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