Title:
ＲＮＡの安定化
Document Type and Number:
Japanese Patent JP7163186
Kind Code:
B2
Abstract:
The present invention pertains to methods, uses, compositions and kits for treating RNA at conditions comprising a temperature of at least 70° C. and a pH of at least 8, preferably a pH of at least 9. It also pertains to methods, uses and compositions for stabilizing RNA at conditions comprising a temperature of at least 70° C. and a pH of at least 8, preferably a pH of at least 9. It based on the finding that RNA can be stabilized in the presence of ammonium sulfate. In particular, the present invention provides a method of processing RNA, comprising treating the RNA at conditions comprising a temperature of at least 70° C. and a pH of at least 8, preferably a pH of at least 9, in the presence of ammonium sulfate at a concentration of 10 mM or less.
Inventors:
Horlitz, Martin
Earl Muller, Uwe
Earl Muller, Uwe
Application Number:
JP2018548769A
Publication Date:
October 31, 2022
Filing Date:
March 16, 2017
Export Citation:
Assignee:
Qiagen Gamebha
International Classes:
C12N15/10; B01J41/04; B01J41/09; B01J47/018; B01J47/02; B01J47/12; B01J49/07; B01J49/57; C12N15/11
Domestic Patent References:
| JP2015533288A | ||||
| JP2004516853A | ||||
| JP2009518020A | ||||
| JP2013505719A | ||||
| JP63154696A | ||||
| JP2002521071A |
Attorney, Agent or Firm:
Shusaku Yamamoto
Morishita Natsuki
Morishita Natsuki