Title:
RANDOM HOMOZYGOUS GENE PERTURBATION (RHGP) WITH THERMAL ASYMMETRIC INTERLACED (TAIL) PCR
Document Type and Number:
Japanese Patent JP2015096067
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a method for identifying host genes and encoded proteins for therapeutic targets in a short period of time.SOLUTION: The period for identification of promising targets is reduced from months to weeks or less by the combination of Random Homozygous Gene Perturbation (RHGP), that a Gene Search Vector, that is either lentivirus or MMLV-based, is inserted into a cell genome, with Thermal Asymmetric Interlaced (TAIL) PCR for the vector insertion site. RHGP is used to identify potential host targets for influenza, HIV and other viral infections. Specific targets including PTCH1, Robo1 and Nedd4 are reviewed in detail.
Inventors:
KINCH MICHAEL S
GOLDBLATT MICHAEL
LI WU-BO
BAMBA DOUTY
CHANG SHAOJING
CHEN HUOSHENG
FESSEHA ZENBEWORK
KOHLI MANU
MAO HANWEN
UNG-MEDOFF HEATHER THI THU
WENG KE
GOLDBLATT MICHAEL
LI WU-BO
BAMBA DOUTY
CHANG SHAOJING
CHEN HUOSHENG
FESSEHA ZENBEWORK
KOHLI MANU
MAO HANWEN
UNG-MEDOFF HEATHER THI THU
WENG KE
Application Number:
JP2014242887A
Publication Date:
May 21, 2015
Filing Date:
December 01, 2014
Export Citation:
Assignee:
LILLY CO ELI
International Classes:
C12Q1/02; A61K39/395; A61K45/00; A61K48/00; A61P31/16; A61P31/18
Foreign References:
| WO2003075860A2 | 2003-09-18 |
Other References:
JPN6013054862; Ｊ． Ｌｅｕｋｏｃ． Ｂｉｏｌ．， ２００７， ８２（３）， ｐｐ．４６５-４７６
Attorney, Agent or Firm:
Hiroshi Kobayashi
Norio Omori
Saori Oga
Yasuhito Suzuki
Norio Omori
Saori Oga
Yasuhito Suzuki