Title:
薄膜培養デバイスにおける大腸菌の迅速な検出
Document Type and Number:
Japanese Patent JP7125975
Kind Code:
B2
Abstract:
A device for differentially enumerating colonies of coliform and Escherichia coli microorganisms is provided. The device comprises a water-impermeable first sheet; a water-impermeable second sheet attached to the first sheet; a dry, rehydratable culture medium comprising a lactose-fermentation indicator system, a β-D-glucuronidase indicator system that comprises a plurality of compounds that enhance β-glucuronidase enzyme activity in E. coli, a redox indicator system and a first cold-water soluble gelling agent adhered to the first sheet, the culture medium disposed in a microbial growth zone; and a second cold-water-soluble gelling agent adhered to the second sheet. The microbial growth zone is disposed between the first sheet and the second sheet. Methods of using the device are also provided.
Inventors:
Chandrapaty, Sai Raja
Teagarden, Alex Jay.
Sadrith, Halley E.
Teagarden, Alex Jay.
Sadrith, Halley E.
Application Number:
JP2020502536A
Publication Date:
August 25, 2022
Filing Date:
April 02, 2018
Export Citation:
Assignee:
3M Innovative Properties Company
International Classes:
C12M1/34; C12Q1/06
Domestic Patent References:
| JP2014504502A | ||||
| JP2001333795A | ||||
| JP11505405A | ||||
| JP2010517552A | ||||
| JP2002513288A | ||||
| JP8510124A | ||||
| JP2014183802A |
Foreign References:
| US4565783 | ||||
| US5510243 | ||||
| US5605812 | ||||
| WO2012092090A1 |
Other References:
Ann. Microbiol.,2010年,Vol. 60,pp. 373-376
Attorney, Agent or Firm:
Fujii Ken
Taro Akazawa
Wakako Nomura
Tsukuda Seigen
Hideto Asamura
Taro Akazawa
Wakako Nomura
Tsukuda Seigen
Hideto Asamura