Title:
【発明の名称】急速加熱ブロックヒートサイクラー
Document Type and Number:
Japanese Patent JP2002542445
Kind Code:
A
Abstract:
A heat block thermocycler to perform rapid PCR in multiple small-volume samples (0.5-10 mu l) employing a small, low-profile, low thermal capacity sample block the temperature of which can be rapidly and accurately modulated by a single thermoelectric pump. An array of spaced-apart sample wells is formed in the top surface of the block. The samples are placed into the wells of a small, ultrathin-walled (20-50 mu m) multiwell plate and located into the sample block. The multiwell plate closely fits the array of sample wells and the top surface of the sample block. The heated lid tightly seals the wells by pressing the sealing film to the top surface of the multiwell plate supported by the top surface of the sample block. Air pressure arising inside the tightly sealed wells at elevated temperatures deforms the elastic walls of the wells of the ultrathin-walled plate and brings them into close thermal contact with the sample block. An elastic gasket thermally isolates the sample block from the heated lid.
Inventors:
Tretyakov, Alexandre
The Roots, Hans-Peter
The Roots, Hans-Peter
Application Number:
JP2000611719A
Publication Date:
December 10, 2002
Filing Date:
April 05, 2000
Export Citation:
Assignee:
Analytic Jena Age
International Classes:
F25B21/02; B01L3/00; B01L7/00; C12M1/00; C12Q1/68; (IPC1-7): F25B21/02; C12M1/00
Domestic Patent References:
| JPH075180A | 1995-01-10 | |||
| JPH06500727A | 1994-01-27 | |||
| JPH07151764A | 1995-06-16 | |||
| JPH06233670A | 1994-08-23 |
Attorney, Agent or Firm:
Yu Saito (2 outside)