PURPOSE: To readily measure the titled enzyme useful for screening patients suffering from hepatopathy and hematopoietic malignant tumor in blood serum with a high accuracy, by adding an inhibitor capable of simultaneously inhibiting activities of allylamidase and cystylaminopeptidase.
CONSTITUTION: An inhibitor for allylamidase (AA) and cystylaminopeptidase (CAP), e.g. o-phenanthroline, etc., glutamic acid dehydrogenase, reduced form nicotinamide adenine dinucleotide phosphate (NADPH), α-ketoglutaric acid, magnesium chloride and a buffer solution at 7.0W8.0 pH are added to a blood serum specimen and incubated at 37°C for 5W10min. A buffer solution at 7.0W8.0 pH containing L-leucinamide as a substrate for cytosol leucine aminopeptidase (CLAP) is added to measure the reduction of absorbance of the NADPH in the ultraviolet region. Thereby the cytosol leucine aminopeptidase is specifically measured.
SUGIYAMA MASAYASU
SHIMIZU HIROSHI
