組換え大腸菌及びそれを用いたイソプレノイドの製造方法

Title:

組換え大腸菌及びそれを用いたイソプレノイドの製造方法

Document Type and Number:

Japanese Patent JP5405030

Kind Code:

B2

Abstract:

To provide a method for efficiently producing isoprenoids using Escherichia coli.

Provided are a recombinant Escherichia coli obtained by transferring and expressing (1) the gene or gene group of an enzyme for synthesizing isoprenoids from farnesyl diphosphate, (2) type 2 isopentenyl diphosphate isomerase gene, and (3) a mevalonic acid route gene group for synthesizing isopentenyl diphosphate from acetoacetyl-coenzyme A; and the method for producing the isoprenoids, characterized by culturing the recombinant Escherichia coli in a culture medium containing acetoacetate and then obtaining the isoprenoids from the culture product or the bacterial cells.

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Inventors:

Naoshi Harada
Norihiko Misawa
Ryutaro Utsumi

Application Number:

JP2008051539A

Publication Date:

February 05, 2014

Filing Date:

March 03, 2008

Export Citation:

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Assignee:

Kirin Holdings Company, Limited
Kinki University

International Classes:

C12N15/09; C12N1/21; C12P5/00; C12P23/00

Domestic Patent References:

JP2004236618A

Other References:

Planta,2008年 2月14日,Vol.227,p.1291-1299
J Am Chem Soc,2001年,Vol.123,p.1238-1239
Biochem J,1997年,Vol.324,p.421-426
Appl Microbiol Biotechnol,2009年,Vol.81,p.915-925

Attorney, Agent or Firm:

Kenichi Nomura
Setuko Mayama

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