Title:
組換え北アメリカ1型ブタ生殖器呼吸器症候群ウイルス及び使用方法
Document Type and Number:
Japanese Patent JP2010531143
Kind Code:
A
Abstract:
Porcine reproductive and respiratory syndrome virus (PRRSV) is a major problem in the pork industry worldwide. The inclusion of markers in vaccines will allow for diagnostic differentiation of vaccinated animals from those naturally infected with wild-type virus. Using a cDNA infectious clone of North American Type 1 PRRSV, a recombinant green fluorescent protein (GFP) tagged PRRSV has been made, containing deletion of an immunogenic epitope, ES4, in the nsp2 region. GFP and ES4 epitope-based ELISAs compliment the marker identification.
Inventors:
Fang, Inn
Hennings, Jane
Nelson, Eric A.
Hennings, Jane
Nelson, Eric A.
Application Number:
JP2010513497A
Publication Date:
September 24, 2010
Filing Date:
June 24, 2008
Export Citation:
Assignee:
SOUTH DAKOTA STATE UNIVERSITY
International Classes:
C12N7/06; A61K39/12; A61P11/00; A61P15/06; C12N15/09; G01N33/53
Attorney, Agent or Firm:
Mieko Kashihara
Goichi Takahashi
Masahito Shibata
Goichi Takahashi
Masahito Shibata